Análise do processo de testagem e gestão laboratorial para o exame de carga viral de HIV-1 no sector laboratorial de biologia molecular do Hospital Geral de Mavalane

Abstract

Currently, combined antiretroviral therapy (cART) is the main strategy for recovering the immune response, providing a reduction in the occurrence of opportunistic diseases and increasing the survival of people living with HIV (PLHIV). However, the rate of diagnosis of HIV infection in the advanced stage of immunosuppression is still very high in low-income countries with poor health literacy, as is the case in Mozambique. HIV viral load is also indicative of the risk of HIV transmission, and randomized controlled clinical trials have established that early initiation of ART with viral load suppression reduces HIV transmission by 96%. This report concerns the internship that aimed to analyze the Testing and Laboratory Management Process for HIV-1 Viral Load Testing in the Molecular Biology Laboratory, which is aligned with the ISO 15189:2022 standard, which defines requirements for quality and competence in diagnostic laboratories. The internship allowed for a range of activities, such as Disinfection of workbenches and Completion of temperature and decontamination control forms, Reception of samples, Verification of sample conformity, Registration of samples in the DISA system, Cutting of PSC samples, Elution, Disposal of PSC samples after processing, Periodic maintenance of biosafety cabinets, Daily and periodic maintenance of the Cobas 6800 equipment, Organization of liquid plasma samples, Error handling and repetitions, Completion of the control form for processed samples, Validation of reagent batches and preventive maintenance, Validation of batch results with controls within limits and repetition of samples with invalid controls, Disposal of samples with invalid results and final screening of samples.(TRADUÇAO NOSSA)