Criação de um método simples de pesquisa de compostos com propriedades antioxidantes de origem vegetal

Abstract

The present work is basically aimed at creating a simple method for researching compounds with plant-derived antioxidant properties. It has an initial part of bibliographic study that includes a survey of the characteristics of antioxidant compounds, their mechanism of action, classes of compounds with this type of properties and main natural sources, as well as their geographic location in Mozambique. During the study, attention is paid to the existing methods of researching them, the difficulties and disadvantages in applying each method, and the type of equipment involved in the experiments. The experimental section is divided into two parts: The first includes extraction using the appropriate solvents according to the class of compounds to be extracted, carrying out thin layer and medium pressure column chromatography (MPLC) to follow the work process and carrying out qualitative tests to search for antioxidant compounds . In the second, optimal conditions are created (temperature and oxygen pressure) for the oxidation of linoleic acid. Two blank tests are carried out, a known antioxidant is applied at different concentrations to test the validity of the method and finally plant extracts are applied to measure their antioxidant power. A statistical analysis of the results obtained is performed by comparing some parameters. Based on the theoretical and statistical interpretation of the results, it is concluded that the method created is valid for the research of compounds with antioxidant properties of plant origin. (TRADUÇÃO NOSSA)